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KMID : 1098420030110040311
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Korean Journal of Medicinal Crop Science
2003 Volume.11 No. 4 p.311 ~ p.315
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Comparative Cytogenetic Characteristics and Physical Mapping of the 17S and 5S Ribosomal DNAs between Atractylodes japonica Koidz, and Atractylodes macrocephala Koidz
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Bang, Kyong Hwan
Koo, Dal Hoe/Kim, Hong Sig/Song, Beom Heon/Cho, Yong Gu/Cho, Joon Hyeong/Bang, Jae Wook
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Abstract
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This study was carried out to compare chromosomal characteristics between Atractylodes japonica and A macmcephala. Cytogenetic analysis was conducted based on karyotype analysis and physical mapping using fluorescence in situ hybridization. As a result of karyotype analysis by feulgen staining, somatic chromosome numbers of A japonica and A macrocephala were 2n=24. The length of the mitotic metaphase chromosomes of A japonica ranged from 0.70 to 1.60 Pl with a total length of 12.11 ㎛ and the homologous chromosome complement comprised six metacentrics, five submetacentrics and one subtelocentrics. On the other hand, the length of the mitotic metaphase chromosomes of A macrocephala ranged from 0.90 to 2.35 ㎛ with a total length of 16.58 PI and the homologous chromosome complement comprised seven metacentrics and five submetacentrics. The total length of A japonica chromosomes was shorter than that of A macrocephala, but A japonica had one subtelocentrics (chromosomes 4) different from A macrocephala chromosomes. The FISH technique using 17S and 5S rDNA was applied to metaphase chromosomes. The signals for 17S rDNA were detected on the telomeric regions of chromosomes 4 and 5 in both A japonica and A macrocephala. The 5S rDNA signal was found in the short arm of chromosome 1.
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